P003 Ni NTA μSphereP003
亲和层析填料 纯化介质
产品概述:
P003 NTA-μSphere
镍螯合层析填料,用于纯化His-Tag蛋白
affinity purification matrix for His-Tag proteins. polymer uSphere-based chromatographic matrix with High Flow Rate. High capacity and high performance.
NTA-mSphere is a nickel Affinity resin used for the purification of histidine-tagged proteins. The unique, non-charged, hydrophilic linkage of the NTA chelate group to the mSphere ensures high selectivity for histidine-tagged proteins and reduced non-specific binding of other proteins. Its high selectivity allows for one-step purification. Affinity gel can be used in either denaturing or non-denaturing conditions.
IDA or NTA? Briefly, NTA chelates metal ion tighter that IDA and metal leakage low, but difficult to elute the target proteins. IDA chelates metal ion loosely and metal leakage is more but easier to elute the target proteins. Please see “NTA vs. IDA- a tale of two ligands” for details.
产品内容
PAE μSphere™层析填料基材简介 μSphere™-Based Chromatography Media
A New Generation of Matrix for Protein Purification Offers 3 High (3H)
A New Standard and Criteria for Chromatography Media
μSphere™微球是与Source相同的层析填料基材，是集高流速、高载量、高分辨率(3H)于一体的新一代蛋白纯化填料的基础材料。过去二十多年来，聚合物微球得到了极大发展并成功应用于生物物质分离和纯化。PAE开发的聚合物微球，简写μSphere™是用单分散高交联度聚丙烯酸酯微球为基材并在其表面加特殊涂层并接枝功能基团或配体制备的层析介质。传统的多糖基基材虽生物相容性好，但其骨架刚性低，在较高的压力下容易出现变形或者孔道的改变，影响纯化效率。μSphere™有效解决了琼脂糖骨架刚性低的问题，具有诸多更适于线性放大，加快工艺开发速度。不但适于实验室研究用样品的制备，而且是工业规模纯化蛋白、多肽、核酸、抗生素和低分子量药物不可回避的选择，且 PAE生产的用于生物大分子分离的微球为多孔的，有多种粒径和孔径，最大可达1000A,根须需求接上-OH,-COOH或-NH2 等基团，一边方便偶联其它配体或配基。粒径/孔径默认50mm，粒径800 Å。可按客户要求提供粒径15mm，30mm，100mm和孔径300、500和800的产品。
高流速
μSphere具有很高的机械稳定性且粒径单一，因此层析特性较好，具有极很好的抗压缩性能，不易出现堵塞。另外，其可耐操作过程中高至10MPa的压力，支持高达2000cm/hour的流速。
高载量
μSphere是一种可精确控制孔径和粒径的单分布多孔微球体，其比表面积高达>500m2/g,用其制备的层析填料具有很高的功能基团密度，其动态载量极高。图1是NTA-μSphere和NTA-Sepharose FF对比15个His-标签蛋白的载量分布。图中蓝色柱代表NTA-μSphere对某样品的最大载量占NTA-Sepharose FF最大载量的倍数，红线是NTA-Sepharose FF的相对载量等高线，可见NTA-μSphere显著超过NTA-Sepharose FF。
高分辨率
μSphere的粒径相对较小(50μm±5%)且分布相当均一，机械强度高，不易变形，因此分辨率明显高于糖基微球。图2是NTA-μSphere和NTA- Sepharose FF上样量等条件完全均一条件下的洗脱峰，显然，NTA-μSphere的峰形更”sharp”。
其他特点
除上述3H外，mSphere在pH1到14都是稳定的，因此可以用0.5 MNaOH甚至更高的碱浓度进行在位清洗(CIP)。碱液在位清洗是医用产品除热源以及杂蛋白的方法。
Appendix
下表是mSphere与Agarose微球与蛋白纯化有关的理化性质的比较。从中可以看出，mSphere具有多方面的优势。
主要基材类型总结与对比
| 类型 | 基材 | 粒径(μm） | pH稳定性 | 压力 | 流速(cm/h) |
| Source 15 | PS/DVB | 15 | 1~14 | 10MPa | 900 |
| Source 30 | PS/DVB | 30 | 1~14 | 10 | 2000 |
| Resource | PS/DVB | 15 | 1~14 | 10 | 1800 |
| MonoBeads | Monodisperse porous PS/DVB | 10 | 1~14 | 10 | 1200 |
| MiniBeads | Monodisperse PS/DVB | 3, non porous | 1~14 | 10 | |
| Fast Flow | 6% cross-linked agarose | 45-165, avr. 90 | 2~13 | 0.3 | 300 |
| Capto | Highly cross-linked agarose with dextran surface extender | 90 (d50V) | 2~13 | 0.3 | 1200 |
| HP | 6% cross-linked agarose | 24-44, avr. 34 | 1~14 | 0.3 | 150 |
| mSphere | Polyacrylate or PS/DVB | 3, 10, 15, 30, 50, 100 | 1~14 | 10 | 1200~2000 |
P003 NTA-μSphere
镍螯合层析填料,用于纯化His-Tag蛋白
affinity purification matrix for His-Tag proteins. polymer uSphere-based chromatographic matrix with High Flow Rate. High capacity and high performance.
NTA-mSphere is a nickel Affinity resin used for the purification of histidine-tagged proteins. The unique, non-charged, hydrophilic linkage of the NTA chelate group to the mSphere ensures high selectivity for histidine-tagged proteins and reduced non-specific binding of other proteins. Its high selectivity allows for one-step purification. Affinity gel can be used in either denaturing or non-denaturing conditions.
IDA or NTA? Briefly, NTA chelates metal ion tighter that IDA and metal leakage low, but difficult to elute the target proteins. IDA chelates metal ion loosely and metal leakage is more but easier to elute the target proteins. Please see “NTA vs. IDA- a tale of two ligands” for details.
技术参数：
| Particle Size (mm) | Pore Size (Å) | Ligand Density | Target Protein Binding Capacity (g/ml) | Pressure(MPa) | pH Stability | SuggestedFlow Rate（cm/h） | Matrix |
| 50 | 800 | 10 | 3~13 | 150-700 | Polyacrylate |
其他名称：
Ni-NTA Agarose, Ni-NTA-Sepherose, Ni-NTA Agarose, Ni-NTA Sehperose, Chelating Sepherose, Ni-IDA Agarose, Ni-IDA -Sepherose, Ni-IDA Agarose, Ni-IDA Sehperose, Chelating Sepherose, Ni sepharose high performance, Ni-亲和树脂，Ni-亲和层析凝胶，Ni-亲和层析填料, IMAC-Sepherose, IMAC-Agarose,Ni Agarose, Ni-agarose, Ni-Sepherose, Ni Sepherose, Ni琼脂糖，镍琼脂糖凝胶，镍-琼脂糖凝胶，镍亲和凝胶, IDA-Agarose, IDA Sepherose, ida nta, ida-nta, IDA-μSphere，NTA-μSphere, IDA μSphere，NTA μSphere, IDA聚合物微球，NTA聚合物微球, IDA-聚合物微球，NTA-聚合物微球, IDA-BestRose，NTA-BestRose, IDA BestRose，NTA BestRose, Ni Sepharose 6 Fast Flow, 17-5318-01, 17-5318-02, 17-5318-03, 17-5318-04, 17-5318-05, Ni Sepharose High Performance, 17-5268-02, 17-5268-01, 17-5268-03, 17-5268-024,
