Protein AG'-μSphere is an affinity resin with a genetically modified recombinant Protein AG' fusion protein joins IgG binding domains of both Protein A and Protein G. Protein AG. The binding dependency to pH of Protein AG' lower than Protein A, but has the additive properties of Protein A and G together. Protein AG' binds to all subclasses of human IgG, making it helpful for purifying polyclonal or monoclonal IgG antibodies whose subclasses have not been identifieed. Protein AG binds to IgA, IgE, IgM and IgD. Protein AG binds to all subclasses of mouse IgG excluding mouse IgA, IgM or serum albumin. This permits Protein AG to be used in purification and detection of mouse monoclonal IgG antibodies, with no interference from IgA, IgM and serum albumin. Mouse monoclonal antibodies normally have a stronger affinity to the chimeric Protein AG than to either Protein A or Protein G. Protein AG also has been used for purification of macaque IgG.
This product is a matrix with  Protein AG' , an genetically modified alkaline molecule which is recombinantly expressed in E. coli and immobilized to μSphere

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