产品内容
P003 NTA-μSphere
Affinity purification matrix for His-Tag proteins. polymer uSphere-based chromatographic matrix with High Flow Rate. High capacity and high performance.
NTA-mSphere is a nickel Affinity resin used for the purification of histidine-tagged proteins. The unique, non-charged, hydrophilic linkage of the NTA chelate group to the mSphere ensures high selectivity for histidine-tagged proteins and reduced non-specific binding of other proteins. Its high selectivity allows for one-step purification. Affinity gel can be used in either denaturing or non-denaturing conditions.
IDA or NTA? Briefly, NTA chelates metal ion tighter that IDA and metal leakage low, but difficult to elute the target proteins. IDA chelates metal ion loosely and metal leakage is more but easier to elute the target proteins. Please see “NTA vs. IDA- a tale of two ligands” for details.
技术参数：
| Particle Size (mm) | Pore Size (Å) | Ligand Density | Target Protein Binding | Pressure | pH Stability | Suggested | Matrix |
| 50 | 800 | ~150mm Ni | 5~90 mg of a His-tagged | 10 | 3~13 | 150-700 | Polyacrylate |
其他名称：
Ni-NTA Agarose, Ni-NTA-Sepherose, Ni-NTA Agarose, Ni-NTA Sehperose, Chelating Sepherose, Ni-IDA Agarose, Ni-IDA -Sepherose, Ni-IDA Agarose, Ni-IDA Sehperose, Chelating Sepherose, Ni sepharose high performance, Ni-亲和树脂，Ni-亲和层析凝胶，Ni-亲和层析填料, IMAC-Sepherose, IMAC-Agarose,Ni Agarose, Ni-agarose, Ni-Sepherose, Ni Sepherose, Ni琼脂糖，镍琼脂糖凝胶，镍-琼脂糖凝胶，镍亲和凝胶, IDA-Agarose, IDA Sepherose, ida nta, ida-nta, IDA-μSphere，NTA-μSphere, IDA μSphere，NTA μSphere, IDA聚合物微球，NTA聚合物微球, IDA-聚合物微球，NTA-聚合物微球, IDA-BestRose，NTA-BestRose, IDA BestRose，NTA BestRose, Ni Sepharose 6 Fast Flow, 17-5318-01, 17-5318-02, 17-5318-03, 17-5318-04, 17-5318-05, Ni Sepharose High Performance, 17-5268-02, 17-5268-01, 17-5268-03, 17-5268-024
