脱盐时, G 25,G50如何选择?
G25和G50的区别是孔径有明显差异。G50的孔径较大。更适宜处理分子量较大的目标蛋白,如抗体等等。
据此建议:当目标蛋白的分子量大于1500kda时应当用G50。其他情况用G25。根据我们的测试。总体来说,G25的脱盐效果更高一些。因此,在对脱盐效果要求较高时尽量使用G25。用G25处理大分子量蛋白时可能会导致流速低,操作时间拉长。这种情况对于脱盐效果有一定的影响。但通常还是可以获得理想的结果的。
PDX DESALTING COLUMN SERIAL
PDX脱盐柱采用PAE研发制造高品质A型层析柱空柱装填,具有A型柱具有的所有在应用上的便利。
本系列有PAE自产胶和GE原装胶两类供用户选择。
本系列有2.5, 4.5, 8.3, 25和30ML4种胶床体积,可满足0.75至9ML上样量的需要。其中,PD10与GE的PD-10 DESALTING COLUMN胶体积相同。
编号 | 产品名称 | 标示容积 (ML) | 理论容积 (ML) | 胶体积 (ML) | 脱盐上样量范围 | 自产胶预装柱价格 | ||
中间 | ||||||||
PC DS-A2.5 | PD2.5 DESALTING COLUMN | 3 | 3.94 | 2.5 | 0.625 | 0.75 | 1 | 75 |
PC DS-4.5 | PD4.5 DESALTING COLUMN | 5 | 6.37 | 4.5 | 1.125 | 1.35 | 1.8 | 120 |
PC DS-8.3 | PD10 DESALTING COLUMN | 8.3 | 13.71 | 8.3 | 2.075 | 2.49 | 3.32 | 180 |
PC DS-25 | PD25 DESALTING COLUMN | 30 | 37.37 | 25 | 6.25 | 7.5 | 10 | 450 |
PC DS-30 | PD30 DESALTING COLUMN | 55 | 78.68 | 30 | 7.5 | 9 | 12 | 488 |
ABOUTPDX DESALTING
HANDLES SAMPLE VOLUMES OF 1.5 ML, OR LARGER VOLUMES BY CONNECTING UP TO FIVE COLUMNS IN SERIES.
REPLACES DIALYSIS AS THE METHOD OF CHOICE FOR DESALTING.
DESALTING REQUIRES LESS THAN ONE MINUTE DUE TO THE HIGH RIGIDITY OF SEPHADEX G-25 AND THE OPTIMIZED PACKING TECHNOLOGY.
PDX DESALTING IS A PREPACKED, READY TO USE COLUMN FOR GROUP SEPARATION BETWEEN HIGH AND LOW MOLECULAR WEIGHT SUBSTANCES, I.E., BUFFER EXCHANGE PRIOR TO OR AFTER DIFFERENT CHROMATOGRAPHIC STEPS, REMOVAL OF LOW MOLECULAR WEIGHT CONTAMINANTS OR REMOVAL OF REAGENTS TO TERMINATE A REACTION
SEPHADEX G-25 IS WELL ESTABLISHED GEL FILTRATION MEDIUM FOR DESALTING AND BUFFER EXCHANGE IN INDUSTRIAL APPLICATIONS
QUICKLY DESALTS, REMOVES CONTAMINANTS AND TRANSFERS TO A NEW BUFFER IN A SINGLE STEP.
EXCELLENT RECOVERY AND MINIMUM SAMPLE DILUTION
AVAILABLE IN PREPACKED HITRAP DESALTING COLUMNS FOR FAST AND CONVENIENT DESALTING
BIOPROCESS MEDIUM SUPPORTED FOR INDUSTRIAL APPLICATIONS AND WELL-ESTABLISHED FOR DESALTING AND BUFFER EXCHANGE IN INDUSTRIAL APPLICATIONS
SEPHADEX IS A GEL FILTRATION MEDIUM PREPARED BY CROSSLINKING DEXTRAN WITH EPICHLOROHYDRIN. DIFFERENT TYPES OF SEPHADEX DIFFER IN THEIR DEGREE OF CROSS-LINKING AND HENCE IN THEIR DEGREE OF SWELLING AND THEIR MOLECULAR FRACTIONATION RANGE. SEPHADEX G-25 IS ONE OF FIVE DIFFERENT G-TYPES RANGING FROM G-10 FOR SMALL MOLECULES TO G-75 FOR LARGER MOLECULES. SEPHADEX G-50 IS AVAILABLE IN 4 DIFFERENT PARTICLE SIZES (COURSE, MEDIUM, FINE & SUPERFINE).
SEPHADEX G-25 IS AVAILABLE IN DIFFERENT BULK PACK SIZES AND CONVENIENT PRE-PACKED FORMATS.
AS A BIOPROCESS MEDIUM, SEPHADEX G-50, MEETS INDUSTRIAL DEMANDS WITH SECURITY OF SUPPLY AND COMPREHENSIVE TECHNICAL AND REGULATORY SUPPORT.