脱盐时, G 25,G50如何选择?
G25和G50的区别是孔径有明显差异。G50的孔径较大。更适宜处理分子量较大的目标蛋白,如抗体等等。
据此建议:当目标蛋白的分子量大于1500kda时应当用G50。其他情况用G25。根据我们的测试。总体来说,G25的脱盐效果更高一些。因此,在对脱盐效果要求较高时尽量使用G25。用G25处理大分子量蛋白时可能会导致流速低,操作时间拉长。这种情况对于脱盐效果有一定的影响。但通常还是可以获得理想的结果的。
HIPREPX DESALTING COLUMNS
直连GE等的HPLC,亦可重力
默认柱子是链接蠕动泵,要链接AKTA请备注!!
本产品采用PAE自产的B型系列空柱装填SEPHADEX G25或G50脱盐填料而成。和B型柱一样,可以直连AKTA,也可以用重力、离心、蠕动泵等方式操作。特别提醒:本系列产品可以配用PAE自产的上样杯,使操作更为方便快捷。8ML和27ML两种可以以50ML离心管为接收管进行离心操作。具体方法:8ML柱:(1)按照PDX脱盐柱的过程完成平衡、上样。(2)在1只50ML离心管内放置1只合适的接收管,如5ML离心管。把前述上样的柱子放到50ML离心管内并使其出口端插入到接收管中。然后,按照PDX柱的说明在50ML离心转头上离心。27ML柱:用8ML柱的方法完成上样。卸掉层析柱的盖子后放入50ML离心管。
HiPrep Desalting is a serial of convenient, easy-to-use column prepacked 8,27,45,70,100,150,180,220,318,580 ml columns with Sephadex G-25 Fine for desalting and buffer exchange.
n Provide reliable and reproducible desalting and buffer exchange with sample sizes of up to 175 ml sample per column.
n Replaces dialysis for desalting and therefore saves time and reduces labor.
n Compatible with a laboratory pump or chromatography system such as ÄKTA design or equivalents.
n Two or four columns can be used in series for sample sizes of 300 to 600 ml.
Table 1 Characteristics of HiPrep Desalting Column from pae | ||||||||
Cat No | Product Name | Bed Vol (ml) | Void Vol (ml) | Dimensions (mm) | 脱盐上样量范围 | 价格 | 工作模式 | |
PC DS-B8ml | HiPrep8 Desalting Column | 8 | < 0.2 | 12×114 | 2 | 2.4 | 154 | 重力、蠕动泵、Akta、离心 |
PC DS-B27ml | HiPrep27 Desalting Column | 27 | < 0.5 | 21×135 | 6.75 | 8.1 | 464 | 重力、蠕动泵、Akta、离心 |
PC DS-B45ml | HiPrep45 Desalting Column | 45 | < 1 | 21×184, | 11.25 | 13.5 | 760 | 重力、蠕动泵、Akta |
PC DS-B70ml | HiPrep70 Desalting Column | 70 | < 2 | 26.8×127 | 18.75 | 22.5 | 1,250 | 重力、蠕动泵、Akta |
PC DS-B100ml | HiPrep100 Desalting Column | 100 | < 2 | 39×131 | 25 | 30 | 1,680 | 重力、蠕动泵、Akta |
PC DS-B150ml | HiPrep150 Desalting Column | 150 | < 2 | 31×257 | 37.5 | 45 | 2,275 | 重力、蠕动泵、Akta |
PC DS-B180ml | HiPrep180 Desalting Column | 180 | < 2 | 60×110 | 45 | 54 | 2,734 | 重力、蠕动泵、Akta |
PC DS-B220ml | HiPrep220 Desalting Column | 220 | < 2 | 39×262 | 55 | 66 | 2,966 | 重力、蠕动泵、Akta |
PC DS-B385ml | HiPrep385 Desalting Column | 385 | < 3 | 61×224 | 79.5 | 95.4 | 4,261 | 重力、蠕动泵、Akta |
PC DS-B580ml | HiPrep580 Desalting Column | 580 | < 3 | 61×280 | 6525 | 174 | 7,654 | 重力、蠕动泵、Akta |