HITALON SP ΜSPHERE
HiTrap SμSphere is prepacked with SP μSphere, and is a strong cation exchanger for small-scale protein purifications as well as screening of binding and elutions conditions.
l Convenient and affordable for fast, easy ion exchange separations either alone or connected in series.
l The industry standard for ion exchange chromatography.
l High flow rates and good scale-up potential.
l Use a weak ion exchanger if the selectivity of a strong ion exchanger is insufficient.
l Predictable scale-up
Q, SP, DEAE adn CM μSphere are based on a robust, highly cross-linked monodispersed beads with high flow rates, high loading capacities and high resolution .their performance is much more better that Sepharose based on highly cross-linked beaded agarose.
特点:
l 聚合物为基材的层析填料装填,层析性能显著优于FF。
l Akta标准接口预装柱,尺寸与HiTrap一致,直连Akta。
l 精心设计,精密制造,无死体积
l 适用于GE、Agilent,waters等公司的层析设备,包括但不限于Acta、Avant、Prim。
l 经转接可与BioRad等公司的标准鲁尔接口层析设备(如,NGC等)连接。
l 可以与注射器、蠕动泵或做重力柱使用,充分利用其无死体积的优势。
About * SP μSphere
SP μSphere is a strong cation exchanger based on the well established Sepharose Fast Flow ion exchange platform, extensively used for preparative protein separations in both research and industrial applications.
l Well-proven strong cation exchanger developed for industrial downstream processes.
l Used extensively for capture and intermediate purification of a wide range of approved biopharmaceuticals
l The industry standard for ion exchange chromatography during recent decades
l High chemical stability allows for well proven CIP and sanitization protocols
l The hydrophilic nature of the base matrix ensures low levels of non-specific binding leading to low levels of host cell-derived impurities in the elution pool.
SP μSphere is part of the Sepharose Fast Flow ion exchange platform, which has been the industrial standard for ion exchange chromatography during recent decades. It is composed of cross-linked, 6% agarose particles, with sulphopropyl (SP) strong cation exchange groups. SP μSphere has high chemical stability, allowing well proven cleaning-in-place (CIP) and sanitization protocols. Scale-up with SP μSphere is straight forward and the resin is available in a range of pre-packed process development tools.
As member of the BioProcess resin range, SP μSphere meets industrial demands with security of supply and comprehensive technical and regulatory support.
About * SP μSphere
SP μSphere is a strong cation exchanger based on the well established Sepharose Fast Flow ion exchange platform, extensively used for preparative protein separations in both research and industrial applications.
l Well-proven strong cation exchanger developed for industrial downstream processes.
l Used extensively for capture and intermediate purification of a wide range of approved biopharmaceuticals
l The industry standard for ion exchange chromatography during recent decades
l High chemical stability allows for well proven CIP and sanitization protocols
l The hydrophilic nature of the base matrix ensures low levels of non-specific binding leading to low levels of host cell-derived impurities in the elution pool.
SP μSphere is part of the Sepharose Fast Flow ion exchange platform, which has been the industrial standard for ion exchange chromatography during recent decades. It is composed of cross-linked, 6% agarose particles, with sulphopropyl (SP) strong cation exchange groups. SP μSphere has high chemical stability, allowing well proven cleaning-in-place (CIP) and sanitization protocols. Scale-up with SP μSphere is straight forward and the resin is available in a range of pre-packed process development tools.
As member of the BioProcess resin range, SP μSphere meets industrial demands with security of supply and comprehensive technical and regulatory support.