Butyl μSphere is a well established, standard aliphatic hydrophobic interaction chromatography (HIC) resin for capture and intermediate purification of larger proteins.
Standard aliphatic HIC resins based on μSphere base matrix derivatized via uncharged, chemically-stable eth er linkages.Optimized for the separation of larger proteins in capture and intermediate purification stepsBioProcess resin supported for industrial applications and well-established in approved processesThe hydrophilic nature of the base matrix ensures low levels of non-specific binding.
Butyl μSphere is part of the μSphere HIC platform, which has been an industrial standard for HIC chromatography during recent decades.
ButylμSphere is based on cross-linked 4% polymer beads. The aliphatic ligand is immobilized to the base matrix with an ether linkage. The ligand contains no charged groups, making true hydrophobic interaction chromatography possible, without interfering ionic effects.
ButylμSphere is available in a range of different bulk pack sizes and convenient pre-packed formats for easy scale-up and process development.
As member of the BioProcess resins range, Butyl μSphere meets industrial demands with security of supply and comprehensive technical and regulatory support.
技术参数:
Particle Size (mm) | Pore Size (Å) | Ligand Density | Target Protein Binding Capacity (g/ml) | Pressure (MPa) | pH Stability | Suggested Flow Rate(cm/h) | Matrix |
50 | 800 | 53mM Butyl | 27mg BSA | 10 | 3~13 | 150-700 | Polyacrylate |