为什么要选用pae的层析填料？

历史上，琼脂糖微球曾以其规则的球状体带来的高流速、良好的化学稳定性和耐碱性取代了流速极慢，不能耐受0.1M以上NaOH在位清洗，寿命短且难以用于下游纯化的纤维素为基材的层析填料。

pae生产的所有蛋白纯化填料都是以聚合物微球uSphere（中文：妙球）为基材的新一代高性能层析填料，具有3H（高流速、高载量、高分辨）三大突出特征。与琼脂糖微球为代表的多糖基材层析填料。相比，其层析性能可与GE的source系列媲美，与琼脂糖微球相比具有显著优势，而价格只有其几分之一，奢侈品、平民价，性价比突出。

Protein AG' - u Sphere is an affinity resin with a genetically modified recombinant Protein AG' fusion protein joins IgG binding domains of both Protein A and Protein G. ProteinAG. The binding dependency to pH of Protein AG' lower than Protein A, but has the additive properties of Protein A and G together.

Protein AG' binds to all subclasses of humanIgG, making it helpful for purifying polyclonal or monoclonal lgG antibodies whose subclasses have not been identified. Protein AG binds to lgA, IgE, IgM and IgD. Protein AG binds to all subclasses of mouse lgG excluding mouse lgA, IgM or serum albumin. This permits Protein AG to be used in purification and detection of mouse monoclonal lgG antibodies, with no interference from lgA, IgM and serum albumin. Mouse monoclonal antibodies normally have a stronger affinity to the chimeric Protein G than to either Protein A or Protein G. Protein AG also has been used for purification of macaque lgG.

This product is a matrix with Protein AG' an genetically modified alkaline moleculehich is recombinantly expressed in E. coli and immobilized to u Sphere

技术参数：

Particle Size (mm) Pore Size (A) Ligand Density Target Protein Binding

Capacity (mg/ml) Pressure

(MPa) pH Stability Suggested

Flow Rate (cm/h)

Matrix

50 800 >6 um Protein AG 40mg/ml hlgG 10 3^13 150-700 Polyacrylate